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tubes ice mrc 2 crystallization plates  (Eppendorf AG)


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    Eppendorf AG tubes ice mrc 2 crystallization plates
    Tubes Ice Mrc 2 Crystallization Plates, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 27199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tubes+ice+mrc+2+crystallization+plates/Tubes/pm38923271-94-63-62
    Average 99 stars, based on 27199 article reviews
    tubes ice mrc 2 crystallization plates - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Crystallization Assay:

    Article Title: Structure and Stability of Ago2 MID-Nucleotide Complexes: All-in-One (Drop) His 6 -SUMO Tag Removal, Nucleotide Binding, and Crystal Growth.
    Article Snippet: 15 mg/ml His6-SUMO-MID protein (see above) UMP (Sigma, CAS no. 3387-36-8) UTP (Millipore Sigma, CAS no. 19817-92-6) tTTP (2′-3′ linked α-L-threofuranosyl thymidine-3′-triphosphate) (Zhang et al., 2013) H2O, nuclease free (Promega, cat. no. P119E-C) Ulp1 protease (His-tagged SUMO protease, Vanderbilt Center for Structural Biology https:// structbio.vanderbilt.edu/wetlab/private/vectors/Ulp1/Purif. .. Ulp1.pdf ; or ThermoFisher Scientific, cat. no. 12588018) Index screen, 96-condition crystallization kit (Hampton Research, cat. no. HR2-144) Paratone-N oil, Parabar 10312 (Hampton Research, cat. no. HR2-643) Columns for His6-SUMO-MID protein purification (see above): Q sepharose fast flow (GE Healthcare, cat. no. 17-0510-01) SP sepharose fast flow (GE Healthcare, cat. no. 17-0720-01) Superdex 75 10/30 GL (GE Healthcare, cat. no. 17-5174-01) 1.5-ml Eppendorf tubes Ice MRC 2 crystallization plates (Hampton Research, cat. no. HR3-082) Microscope 75-μm MicroMount (MiTeGen, cat. no. M2-) Liquid N2 Crystal imager (Formulatrix imager with UV fluorescence detector) 1. .. In an Eppendorf tube mix His6-SUMO-MID protein with nucleotide ligand, e.g., UMP, UTP, tTTP (in nuclease-free water), in a 1:700 to 1:1000 molar ratio and store the tube on ice for 2 hr.

    Protein Purification:

    Article Title: Structure and Stability of Ago2 MID-Nucleotide Complexes: All-in-One (Drop) His 6 -SUMO Tag Removal, Nucleotide Binding, and Crystal Growth.
    Article Snippet: 15 mg/ml His6-SUMO-MID protein (see above) UMP (Sigma, CAS no. 3387-36-8) UTP (Millipore Sigma, CAS no. 19817-92-6) tTTP (2′-3′ linked α-L-threofuranosyl thymidine-3′-triphosphate) (Zhang et al., 2013) H2O, nuclease free (Promega, cat. no. P119E-C) Ulp1 protease (His-tagged SUMO protease, Vanderbilt Center for Structural Biology https:// structbio.vanderbilt.edu/wetlab/private/vectors/Ulp1/Purif. .. Ulp1.pdf ; or ThermoFisher Scientific, cat. no. 12588018) Index screen, 96-condition crystallization kit (Hampton Research, cat. no. HR2-144) Paratone-N oil, Parabar 10312 (Hampton Research, cat. no. HR2-643) Columns for His6-SUMO-MID protein purification (see above): Q sepharose fast flow (GE Healthcare, cat. no. 17-0510-01) SP sepharose fast flow (GE Healthcare, cat. no. 17-0720-01) Superdex 75 10/30 GL (GE Healthcare, cat. no. 17-5174-01) 1.5-ml Eppendorf tubes Ice MRC 2 crystallization plates (Hampton Research, cat. no. HR3-082) Microscope 75-μm MicroMount (MiTeGen, cat. no. M2-) Liquid N2 Crystal imager (Formulatrix imager with UV fluorescence detector) 1. .. In an Eppendorf tube mix His6-SUMO-MID protein with nucleotide ligand, e.g., UMP, UTP, tTTP (in nuclease-free water), in a 1:700 to 1:1000 molar ratio and store the tube on ice for 2 hr.

    Microscopy:

    Article Title: Structure and Stability of Ago2 MID-Nucleotide Complexes: All-in-One (Drop) His 6 -SUMO Tag Removal, Nucleotide Binding, and Crystal Growth.
    Article Snippet: 15 mg/ml His6-SUMO-MID protein (see above) UMP (Sigma, CAS no. 3387-36-8) UTP (Millipore Sigma, CAS no. 19817-92-6) tTTP (2′-3′ linked α-L-threofuranosyl thymidine-3′-triphosphate) (Zhang et al., 2013) H2O, nuclease free (Promega, cat. no. P119E-C) Ulp1 protease (His-tagged SUMO protease, Vanderbilt Center for Structural Biology https:// structbio.vanderbilt.edu/wetlab/private/vectors/Ulp1/Purif. .. Ulp1.pdf ; or ThermoFisher Scientific, cat. no. 12588018) Index screen, 96-condition crystallization kit (Hampton Research, cat. no. HR2-144) Paratone-N oil, Parabar 10312 (Hampton Research, cat. no. HR2-643) Columns for His6-SUMO-MID protein purification (see above): Q sepharose fast flow (GE Healthcare, cat. no. 17-0510-01) SP sepharose fast flow (GE Healthcare, cat. no. 17-0720-01) Superdex 75 10/30 GL (GE Healthcare, cat. no. 17-5174-01) 1.5-ml Eppendorf tubes Ice MRC 2 crystallization plates (Hampton Research, cat. no. HR3-082) Microscope 75-μm MicroMount (MiTeGen, cat. no. M2-) Liquid N2 Crystal imager (Formulatrix imager with UV fluorescence detector) 1. .. In an Eppendorf tube mix His6-SUMO-MID protein with nucleotide ligand, e.g., UMP, UTP, tTTP (in nuclease-free water), in a 1:700 to 1:1000 molar ratio and store the tube on ice for 2 hr.

    Fluorescence:

    Article Title: Structure and Stability of Ago2 MID-Nucleotide Complexes: All-in-One (Drop) His 6 -SUMO Tag Removal, Nucleotide Binding, and Crystal Growth.
    Article Snippet: 15 mg/ml His6-SUMO-MID protein (see above) UMP (Sigma, CAS no. 3387-36-8) UTP (Millipore Sigma, CAS no. 19817-92-6) tTTP (2′-3′ linked α-L-threofuranosyl thymidine-3′-triphosphate) (Zhang et al., 2013) H2O, nuclease free (Promega, cat. no. P119E-C) Ulp1 protease (His-tagged SUMO protease, Vanderbilt Center for Structural Biology https:// structbio.vanderbilt.edu/wetlab/private/vectors/Ulp1/Purif. .. Ulp1.pdf ; or ThermoFisher Scientific, cat. no. 12588018) Index screen, 96-condition crystallization kit (Hampton Research, cat. no. HR2-144) Paratone-N oil, Parabar 10312 (Hampton Research, cat. no. HR2-643) Columns for His6-SUMO-MID protein purification (see above): Q sepharose fast flow (GE Healthcare, cat. no. 17-0510-01) SP sepharose fast flow (GE Healthcare, cat. no. 17-0720-01) Superdex 75 10/30 GL (GE Healthcare, cat. no. 17-5174-01) 1.5-ml Eppendorf tubes Ice MRC 2 crystallization plates (Hampton Research, cat. no. HR3-082) Microscope 75-μm MicroMount (MiTeGen, cat. no. M2-) Liquid N2 Crystal imager (Formulatrix imager with UV fluorescence detector) 1. .. In an Eppendorf tube mix His6-SUMO-MID protein with nucleotide ligand, e.g., UMP, UTP, tTTP (in nuclease-free water), in a 1:700 to 1:1000 molar ratio and store the tube on ice for 2 hr.



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    tubes ice mrc 2 crystallization plates  (Eppendorf AG)
    99
    Eppendorf AG tubes ice mrc 2 crystallization plates
    Tubes Ice Mrc 2 Crystallization Plates, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tubes+ice+mrc+2+crystallization+plates/Tubes/pm38923271-94-63-62
    Average 99 stars, based on 1 article reviews
    tubes ice mrc 2 crystallization plates - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

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